Purpose
The Analytic Microscopy Core’s (AMC) purpose is to provide access to the latest optical microscopy imaging technologies and expertise. The Core provides training and assisted support for many of its microscopes and imaging analysis software. Core staff members are available to assist investigators in experimental and scientific design of their imaging experiments so that the correct reagents, staining protocols and imaging techniques are employed to best address the scientific questions of the investigator. The AMC staff work closely with members to design, image, and analyze experiments, allowing members to obtain high-quality and reproducible data. Further, the AMC collaborates with other Shared Resource Facilities at MCC to manage complex projects and to ensure proper handoffs of materials and data.
Services
Leica SP5 AOBS Multiphoton Confocal Microscope:
- 9 laser lines and MP Ti-sapphire laser
- 4 PMT and 2HyD detectors
- 3D imaging and reconstruction
- Colocalization assays
- Multiphoton imaging
- FRET & FRAP
- Calcium imaging
- Second Harmonic Generation
Leica SP8 Confocal Microscope:
- 4 laser lines and 2 PMT detectors
- Colocalization assays
- 3D imaging and reconstruction
- HyD detector
- An incubation/hypoxia chamber is available for live cell imaging
Widefield Fluorescence Microscopes:
- Fully automated upright and inverted Zeiss microscopes
- LED light source and detection capabilities of 350-850nm
- Tile scan and z-stack acquisition with post image processing
- Deconvolution
- Fully enclosed incubation chamber for long term time lapse live cell imaging
- Apotome available for pseudo-confocal imaging
- EVOS FL simple observation and acquisition
- EVOS Auto including tile scan and live cell imaging
Intravital Microscopy:
- Live animal imaging via dorsal skin flap or cranial window chambers
- Analyze tumor growth, invasion, collagen or vasculature modeling longitudinally
- Real time analysis of angiogenesis, blood flow, vascular permeability, drug/agent kinetics
- Real time microscopy quantification of pH or other metabolomic gradients
Aperio ScanScope AT2 Slide Scanner:
- Holds up to 400 slides; scan time 30 slides/hour
- Segments TMAs into individual cores
- Eslide manager software capable of positive pixel, nuclear, cell, membrane, and vessel algorithms
- Genie software capable of segmenting regions of interest for IHC analysis
Incucyte Zoom and S3 two color FL systems:
- High throughput live cell microscope with phase contrast and green/red FL
- 96-well Wound Maker for wound healing assays
- Spheroid and Chemotaxis experiment modules
- Various assay kits are available through Essen Bioscience
Image Analysis:
- Definiens Developer
- Definiens Tissue Studio
- Visiopharm
- Imaris
- Image Pro Premier 3D
Also available:
- Laser Capture Microdissection
- Comet Assay Imaging and Analysis
- Stereoscopy
Location
The AMC is located in the Moffitt Research Center (MRC) on the fourth floor, room 4058. An additional satellite facility is located in the Stabile Research Building (SRB) on the ground floor, room 20307. This facility houses the Leica SP5 AOBS Multiphoton Confocal Microscope.
Scheduling
The AMC uses a Laboratory Information Management Suite (LIMS) located on the Moffittnet for all Core requests, scheduling, billing, and usage tracking.
Publications
All publications based on work conducted in the Analytic Microscopy Core should acknowledge the facility. A suggested statement is as follows:
If a Core Staff member(s) significantly contributes or participates in a project, investigators are encouraged to consider including the individual(s) in the list of authors.
Contacts
Scientific Director
Marilyn Bui, MD, PhD
marilyn.bui@moffitt.org
Core Facility Manager
Joseph Johnson
joseph.johnson@moffitt.org
Office: 813-745-8710
Lab Number: 813-745-6157
This work has been supported in part by the Analytic Microscopy Core at the H. Lee Moffitt Cancer Center & Research Institute, a comprehensive cancer center designated by the National Cancer Institute and funded in part by Moffitt’s Cancer Center Support Grant (P30-CA076292)
