Purpose
The Proteomics and Metabolomics Core (PMC) supports investigators with the experience of highly trained staff as well as instrumentation for proteomics and metabolomics studies, including protein, peptide and metabolite separations, MS instrumentation for protein, peptide and metabolite analysis, and data systems, software, and bioinformatics tools for data archiving and analysis. The Proteomics Core performs routine analytical proteomics services, including target discovery, identification and quantitation, and also provides platforms for functional proteomics using a variety of strategies for protein separation, sub-proteome enrichment, post-translational modification analysis (PTM), and quantitation.
Services
Separations / Enrichment:
SDS Gel, Offline UHPLC Separation,Capture for PTM Profiling, Activity-Based Protein Profiling (ABPP)
Sample Clean-up:
SPE, LLE, Peptide Ziptip/Sep-Pak, Size Exclusion, Ultrafiltration
Mass Spectrometry:
GC-MS: Metabolite Analysis
MALDI-MS & MS/MS: Mass Analysis
LC-MS/MS: Protein/Peptide/Metabolite ID, Protein PTM
LC-MRM/LC-PRM: Relative & Absolute Quantification
Quantification: Label Free, Metabolic Labeling, Chemical Labeling
Acknowledgment:
All publications based on work conducted in the Proteomics and Metabolomics Core Facility should acknowledge the facility and include staff in authorship as warranted based on their contributions. A suggested acknowledgment statement is as follows:
"This work has been supported in part by the Proteomics and Metabolomics Core Facility at the H. Lee Moffitt Cancer Center & Research Institute; an NCI designated Comprehensive Cancer Center (P30-CA076292)."
Contacts
Scientific Director
John Koomen, PhD
john.koomen@moffitt.org
Office: 813-745-8524
Core Manager
Bin Fang, PhD
bin.fang@moffitt.org
Office: 813-745-2965
Lab Number
813-745-1192
Lab Location
MRC First Floor – Room 1296
